Congratulations Elisabeth Gau, Dr. Diana Mate and Zhi Zou on their recent Publication!

19/10/2017
 

Sortase-mediated functionalization of microgels with biomolecules

Sortase-mediated functionalization of microgels ACS Publications

Microgels are colloidal macromolecular networks swollen in water while forming stable dispersions. They are highly porous, soft, deformable and exhibit a stimuli-responsiveness in aqueous solutions. Due to their interesting properties, microgels gained much attention in macromolecular and biomedical research fields.

In this work we developed a sortase-mediated method for chemo-selective and efficient decoration of aqueous microgels with biomolecules. Poly(N-vinylcaprolactam) (VCL) microgels with different amounts of glycidyl methacrylate (GMA) as a comonomer incorporated in the microgel shell were synthesized and characterized regarding to their size, swelling degree and temperature-responsiveness in aqueous solutions. The surface of the PVCL/GMA microgel was modified with the specific recognition peptide sequence (LPETG) for Sortase A. Sortase-mediated conjugation of oligoglycin-tagged enhanced Green Fluorescent Protein (eGFP) to the LPETG-modified microgels was successfully performed. Sortase-mediated bioconjugation can be used as a simple and powerful technique for the targeted surface functionalization of stimuli-responsive microgels with biomolecules.

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Gau, E., Mate, D. M., Zou, Z., Oppermann, A., Töpel, A., Jakob, F., Schwaneberg, U. & Pich, A. (2017). Sortase-Mediated Surface Functionalization of Stimuli-Responsive Microgels. Biomacromolecules